Immune status of patients with frontal basilar trauma having bc "syntekost" applied in the surgical treatment. report 1. the state of the systemic cellular and humoral immunity factors of patients with frontal basilar trauma in the acute posttraumatic period prior to surgical treatment.

OBJECTIVE: Introduction: At present biocomposite materials are used in the surgical treatment of frontal bone fracture. They improve osteogenesis, reduce the number of complications. Immunologic aspects of application of these materials are studied insufficiently, therefore this report presents the results of immunoassay of patients with frontal bone fracture in the proximate posttraumatic period before implanting preparation "Syntekost". The aim: To define the role of immune mechanisms in the realization of the biocomposite material's positive influence on the development of effective posstraumatic rehabilitation schemes. PATIENTS AND METHODS: Materials and methods: 16 patients with frontal bone fracture (FBF) were examined on admission to the Otolaryngology Clinics of Vinnitsa Region Hospital. Additionally, 10 patients of the similar age were examined as a control group. The content of cells with markers of surface antigens-CD3,14,16,20,25, concentration of immunoglobulins of classes M,G,A,E, С4 complement component and lactoferrin was determined in blood. Immunoenzyme methods were applied. Nonparametric Wilcoxon - Mann - Whitney test, computer programme WIN Pepi were used for statistical measurements. RESULTS: Results: A decrease in the level of IgM in comparison with practically healthy donors and an increase in the concentration of lactoferrin were identified as humoral immunity factors of patients with frontal basilar trauma. The most significant deviation in the peripheral blood cellular makeup in CD-markers was an increase in cells with markers CD14 and CD16. CONCLUSION: Conclusions: The level of cells and prodefensin-lactoferrin that maintain inborn immunity increases and the concentration of coarse defensive protein decreases in the initial period after frontal bone fracture, which must be taken into consideration during post-surgical treatment.

Local immunity status of oropharynx in patients with scleroma

Introduction: Scleroma is a rare chronic granulomatous disease of the upper respiratory tract caused by Klebsiella pneumoniae subsp. rhinoscleromatis. To date its pathogenesis is as yet little understood. At the same time, scleroma is associated with a number of immune system disturbances. The aim: To study local immunity status of oropharynx in patients with scleroma, and to compare its parameters in various clinical forms of the disease. Material and methods: 20 apparently healthy subjects and 92 patients with scleroma (33 males, 59 females) underwent clinical immunologic evaluation. There were 31 patients with dominating infiltrative form of scleroma, 30 ­ with dominating atrophic form, 31 ­ with dominating scarring form. Concentration of secretory and monomeric immunoglobulin A, immunoglobulin G, α-interferon, interleukin 1ß in oropharyngeal secretion was determined by enzyme immunoassay. Resluts: Patients with scleroma were found to have altered local immunity of oropharyngeal secretion. There was a strong tendency for decreased concentration of secretory immunoglobulin A - 1.3-2.0 times, and decreased immunoglobulin G level ­ 1.5-2.3 times (р < 0.05) as compared to the values in healthy subjects. Specific features of local immunity in oropharyngeal secretion in various forms of scleromatous inflammatory process in upper respiratory tract were found: the most significant decrease of α-interferon concentration in atrophic and scarring forms of the disease, and the largest increase of anti-inflammatory interleukin 1ß and immune complex concentration in infiltrative form of scleroma. Conslusions: The study revealed deficiency of local immunity factors in oropharynx, being indicative of immunopathogenetic role of diagnosed disturbances in development and persistence of chronic inflammation in scleroma, and emphasizing the necessity of immunocorrection in complex therapy of the disease.

Morphological dynamics of bone tissue reparative regeneration during the implantation of biocomposite "syntekost" into the cavity of the traumatic defect of the iliac crest of a rabbit in the experiment.

OBJECTIVE: Introduction: The problem of bone tissue regeneration and the development of the methods of directional influence on bone healing processes are one of the most urgent problems of modern medicine. The question of bone tissue posttraumatic regeneration is particularly important with regard to military conflicts, ecological and technological disasters, and has social and economic relevance, connected with the necessity of long-term treatment and medical rehabilitation. The aim: The objective of the work is the experimental evaluation of bone tissue reparative regeneration during the implantation of biocomposite "Syntekost" (patent for invention №98573 dated 25.05.2012 Ukraine), and аlso dynamics determination of bone tissue reparative regeneration after its application during a year. PATIENTS AND METHODS: Materials and methods: The experiment was carried out on 24 rabbits. All the rabbits were divided into three groups. Penetrating bone damages on the edge of the proximal tibial metaphysis and diaphysis with the diameter of 2,2 mm were formed in the rabbits of the first group using intravenous anesthetic and the dynamics of the reparative regeneration of bone tissue of the iliac crest was studied in 6, 9 and 12 months, without using composite "Syntekost" (control group - 8 rabbits). Penetrating bone damageson the edge of the proximal tibial metaphysis and diaphysis with the diameter of 2,2 mm were formed in the rabbits of the second group and biocomposite "Syntekost" was implantated into them (8 rabbits). Penetrating bone damages on the edge of the proximal tibial metaphysis and diaphysis with the diameter of 2,2 mm were formed in the rabbits of the third group and biocomposite "Syntekost" was implantated into them together with blood plasma and ossein-hydroxyapatite (8 rabbits). RESULTS: Results: In all stages the research showed the significantly higher percentage of splenial bone tissue both in the rabbits that had biocomposite "Syntekost" implanted withoutblood plasma and ossein-hydroxyapatite and in the rabbits that had biocomposite "Syntekost" implanted with blood plasma and ossein-hydroxyapatite in comparison with the one of the rabbits that didn't have biocomposite "Syntekost" implanted after having been injured (р < 0,05). In all stages of the research the rabbits that had biocomposite "Biokost" implanted together with blood plasma and ossein-hydroxyapatite had the significantly higher percentage of neogenic splenial bone tissue and the significantly lower percentage of biocomposite "Syntekost" comparing to the ones of the rabbits that had biocomposite "Syntekost" implanted without blood plasma and ossein-hydroxyapatite (р < 0,05). CONCLUSION: Conclusions: The application of blood plasma and ossein-hydroxyapatite accelerates bone tissue regeneration and the process of biodegradation of biocomposite "Syntekost" throughout the experiment. In all stages of the experiment no toxic influence of biocomposite "Syntekost" on the surrounding bone tissue was found.